LongSAGE during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium. Phanerodontia chrysosporium

White rot fungi are able to degrade woody lignin and other persistent organic compounds including artificial chemicals (e.g. chlorinated dioxin) in secondary metabolism. This ability has potential in a wide range of biotechnological applications including remediation of organopollutants and the industrial processing of paper and textiles. Ligninolytic fungi secondarily secrete extracellular oxidative enzymes thought to play an important role in these compounds decay. However, detail of metabolic pathway and initiation signals of the degradation system is unclear. To investigate genes directly and indirectly related to it, we constructed long serial analysis of gene expression (Long SAGE) library from the most studied white rot fungus, Phanerochaete chrysosporium. Keywords: transcriptome profiling Overall design: To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (LiP) production in Phanerochaete chrysosporium, we constructed the day 3 culture (just started the enzyme production) library and the day 2 culture (the activity of enzymes is not detected) library.

白腐真菌（White rot fungi）能够在次级代谢过程中降解木材木质素及其他持久性有机化合物，包括人工合成化学品（如氯代二噁英（chlorinated dioxin））。该特性在诸多生物技术应用中具备重要潜力，涵盖有机污染物修复以及造纸、纺织行业的工业加工流程。木质素降解真菌会在次级代谢阶段分泌胞外氧化酶，这类酶被认为在上述化合物的降解过程中发挥关键作用。然而，该降解系统的代谢通路及启动信号的具体细节仍未明确。为探究与之直接或间接相关的基因，我们以研究最为深入的白腐真菌——黄孢原毛平革菌（*Phanerochaete chrysosporium*）为实验材料，构建了基因表达长系列分析（Long SAGE, long serial analysis of gene expression）文库。
关键词：转录组谱分析（transcriptome profiling）
整体实验设计：为分析黄孢原毛平革菌在锰过氧化物酶（manganese peroxidase, MnP）与木质素过氧化物酶（lignin peroxidase, LiP）合成启动阶段的转录组谱，我们分别构建了培养第3天（酶合成刚启动）的文库与培养第2天（未检测到酶活性）的文库。