Screening of 2694 RdRP virtual screening hits in RdRP/Nsp7/Nsp8 biochemical assay and confirmation in cellular SARS-CoV-2 assay

This report describes the most relevant results of virtually screening the Janssen Pharmaceutica compound collection for potential activity against SARS-CoV-2 RNA polymerase and confirmation of potential hits in a biochemical SARS-CoV RTC assay and A549-hACE2 cell-based anti-SARS-CoV-2 assay.

本报告详述了针对杨森制药（Janssen Pharmaceutica）化合物库开展虚拟筛选以挖掘潜在抗严重急性呼吸综合征冠状病毒2（SARS-CoV-2）RNA聚合酶活性化合物的核心成果，以及通过生化法严重急性呼吸综合征冠状病毒复制转录复合体（RTC）检测与基于A549-hACE2细胞的抗SARS-CoV-2检测，对上述潜在命中化合物进行验证的相关结果。