Gene expression signatures of cancer-associated fibroblasts in non-small cell lung cancer. Gene expression signatures of cancer-associated fibroblasts in non-small cell lung cancer

To gain insight into the differential gene expression to aberrant fibroblast activation of the TME, we isolated primary CAFs and NFs from human NSCLC tissues and paired normal tissues,and performed microarray screening of CAFs and NFs Overall design: CAFs and NFs were isolated from human NSCLC tissues and paired normal tissues (more than 5 cm away from the tumor margin). Freshly isolated surgical specimens were acquired from three patients. Fibroblast populations were identified by morphology, western blotting and immunofluorescence staining.

为探究肿瘤微环境（Tumor Microenvironment, TME）中成纤维细胞异常激活相关的差异基因表达特征，我们从人类非小细胞肺癌（Non-Small Cell Lung Cancer, NSCLC）组织及其配对正常组织中分离原代癌相关成纤维细胞（Cancer-Associated Fibroblasts, CAFs）与正常成纤维细胞（Normal Fibroblasts, NFs），并对二者开展基因芯片筛查。
实验整体设计：从人类非小细胞肺癌组织及其距肿瘤边缘5cm以上的配对正常组织中分离CAFs与NFs。本次实验的新鲜手术标本来源于3名患者。通过形态学观察、蛋白质印迹（Western Blotting）及免疫荧光染色对成纤维细胞群进行鉴定。