Low-grade glioma microglia

To examine the effect of Nf1 inactivation in astrocytes on normal microglia in the optic nerve, microglia from Nf1 flox/flox (FF) and Nf1 flox/flox; GFAP-Cre (FFC) were flow sorted. High throughput RNA-seq was employed to compare the expression profiles, and upregulated genes in microglia supportive to Nf1 deficient astrocytes were verified. Overall design: Microglia from mice lacking Nf1 in astrocytes were flow sorted. Upregulated genes were verified and further examined.

为探究星形胶质细胞中Nf1失活对视神经内正常小胶质细胞的影响，研究人员通过流式分选获取了Nf1 flox/flox（FF）小鼠与Nf1 flox/flox; GFAP-Cre（FFC）小鼠的小胶质细胞。本研究采用高通量RNA测序（RNA-seq）比较两组小胶质细胞的基因表达谱，并验证了与Nf1缺陷星形胶质细胞相关的小胶质细胞上调基因。实验整体设计：通过流式分选自星形胶质细胞特异性缺失Nf1的小鼠体内获取小胶质细胞，对上调基因进行验证并开展进一步分析。