Toll-Like Receptor 7 Agonist GS-9620 Induces Prolonged Inhibition of HBV in Human Hepatocytes via a Type I Interferon-Dependent Mechanism. Homo sapiens

TLR7 induced transcriptional change in HBV-infected primary human hepatocytes Overall design: RNA-Seq was conducted in two independent primary human hepatocyte (PHH) donors. After day 13 post-infection, primary human hepatocytes (PHH) were infected with HBV and treated with GS-9620, with media from human peripheral blood mononuclear cells (PBMCs) treated with GS-9620 (GS-9620 conditioned media; GS-9620-CM)

Toll样受体7（Toll-like receptor 7, TLR7）诱导乙型肝炎病毒（Hepatitis B Virus, HBV）感染原代人肝细胞的转录变化
实验设计概述：本研究采用两名独立的原代人肝细胞（Primary Human Hepatocyte, PHH）供体开展RNA测序（RNA-Seq）实验。在感染后第13天，对原代人肝细胞（PHH）进行乙型肝炎病毒感染，随后分别以GS-9620以及经GS-9620处理的人外周血单个核细胞（Peripheral Blood Mononuclear Cell, PBMC）条件培养液（GS-9620 conditioned media，简称GS-9620-CM）进行处理。