H4K16ac(L).Clone_8 ChIP-chip

modENCODE_submission_317 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: CME-W1-Cl.8+; Tissue: dorsal mesothoracic disc; Developmental Stage: third instar larval stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line CME-W1-Cl.8+; Antibody H4K16ac(L)

modENCODE_submission_317
本提交源自Gary Karpen主持的modENCODE项目。如需查阅modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）全基因组范围内125种染色体蛋白质及组蛋白修饰的定位位点。本次研究涉及的蛋白质与修饰参与DNA复制、基因表达、基因沉默及遗传等核心染色体功能。我们将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合平铺式基因组芯片技术开展实验。初始阶段将使用三种细胞系与两个胚胎时期的染色质进行定位分析，后续将针对部分蛋白质，将分析拓展至另外四种动物组织/发育时期。
数据使用条款与规范请参阅：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
关键词：ChIP-chip
实验类型：ChIP-chip
生物来源：细胞系：CME-W1-Cl.8+；组织：背中胸盘；发育时期：三龄幼虫期；性别：雄性
重复次数：4
实验因素：细胞系CME-W1-Cl.8+；抗体：H4K16ac(L)