Expression data that were specifically regulated by lncRNA-LFAR1 in mouse primary HSCs. Mus musculus

Long noncoding RNAs (lncRNAs) play important roles in various biological processes; however, few have been identified that regulate hepatic stellate cells (HSCs) activation and the progression of liver fibrosis. Through a detailed analysis of the expression of lncRNAs in various tissues, we discovered the existence of a liver enriched lncRNA-LFAR1 (lncRNA-Liver Fibrosis Associated RNA1). To identify the roles of lncRNA-LFAR1 in liver fiboris, we systematically analyzed the regulation of mRNAs in primary HSCs infected with two separated lnc-LFAR1-shRNAs by RNA-seq, which revealed a panel of mRNAs that were specifically regulated by lncRNA-LFAR1 in mouse primary HSCs. Overall design: To identify the roles of lncRNA-LFAR1 in liver fibrosis, we systematically analyzed the regulation of mRNAs in primary HSCs infected with shRNA-control (n=2), lncRNA-shRNA1 (n=2) and lncRNA-shRNA3 (n=2) by RNA-seq.

长链非编码RNA（Long noncoding RNAs，lncRNAs）在诸多生物学过程中发挥关键调控作用，但目前已被证实可调控肝星状细胞（hepatic stellate cells，HSCs）活化及肝纤维化进展的lncRNAs却为数不多。我们通过对多种组织中的lncRNAs表达谱开展细致分析，发现了一种肝富集型长链非编码RNA——LFAR1（lncRNA-Liver Fibrosis Associated RNA1，即lncRNA-LFAR1）。为明确lncRNA-LFAR1在肝纤维化中的功能，我们借助RNA测序（RNA-seq）技术，系统分析了转染两种独立lnc-LFAR1短发夹RNA（short hairpin RNA，shRNA）的原代肝星状细胞内的mRNA调控情况，最终筛选出小鼠原代肝星状细胞中受lncRNA-LFAR1特异性调控的一系列mRNA。总体实验设计：为明确lncRNA-LFAR1在肝纤维化中的作用，我们通过RNA测序技术，系统分析了转染短发夹RNA对照（shRNA-control，n=2）、lncRNA-shRNA1（n=2）及lncRNA-shRNA3（n=2）的原代肝星状细胞中的mRNA表达调控模式。