MoTrPAC - Endurance Exercise Training Study In 6 Months Old Rats [RRBS]

The goal of the endurance exercise training study in young adult rats was to perform exercise training studies in young adult (6-month-old) F344 rats, and from these rats collect multiple tissues in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia. For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max and training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group. Regular exercise promotes whole-body health and prevents disease, yet the underlying molecular mechanisms throughout a whole organism are incompletely understood. Here, the Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome, and immunome in whole blood, plasma, and 18 solid tissues in Rattus norvegicus over 8 weeks of endurance exercise training. The resulting data compendium encompasses 9466 assays across 19 tissues, 25 molecular platforms, and 4 training time points in young adult male and female rats. Contributor: Molecular Transducers of Physical Activity Consortium (MoTrPAC)

本项针对年轻成年大鼠的耐力运动训练研究，旨在对6月龄年轻成年F344大鼠（F344 rat）开展运动训练实验，并采集多组织样本，为后续化学分析平台的精细化检测提供高质量实验材料。
本研究同步采集10~12只久坐对照组大鼠与8周训练组大鼠的组织样本；8周训练组及其对照组大鼠来源于同一队列，且安乐死时年龄一致。针对年长组大鼠，本研究额外招募了5~6只对照组大鼠，并与1~2周训练组大鼠同步采集样本。

大鼠分为久坐对照组与运动训练组两类。训练组大鼠每周5天在啮齿类动物跑台（rodent treadmill）上进行运动，采用渐进式训练方案，使大鼠运动强度维持在约70%最大摄氧量（VO2max）；训练时段固定为每日10:00至17:00，每周连续训练5天。训练初始阶段，跑台设置为最大摄氧量70%的强度、坡度5°，运动时长20分钟。每日运动时长增加1分钟，直至训练第31天（第7周初期），此时运动时长达到50分钟。由于跑台参数限制，各训练时段的跑速与坡度会以更大幅度提升；训练的最高强度与时长自第31天起固定，并维持至实验最后10天，以确保运动状态达到稳态。若大鼠无法完成每周至少4天的训练，则将其从研究中剔除并实施安乐死。需特别说明的是，跑台初始速度会根据大鼠的性别与年龄进行调整；初始速度与最大速度均基于合格受试大鼠在训练前测试中测得的最大摄氧量结果确定。

对照组大鼠的实验日程与训练组相似：每日将其置于跑台单跑道内运动15分钟，每周5天；跑台速度设置为0 m/min，坡度与同期训练组使用的坡度一致。

规律运动可促进全身健康、预防疾病，但完整机体层面的潜在分子机制仍未完全阐明。本研究依托物理活动分子转导子联盟（Molecular Transducers of Physical Activity Consortium, MoTrPAC），对8周耐力运动训练过程中的挪威褐家鼠（Rattus norvegicus）的全血、血浆及18种实体组织，开展了时序转录组（transcriptome）、蛋白质组（proteome）、代谢组（metabolome）、脂质组（lipidome）、磷酸化蛋白质组（phosphoproteome）、乙酰化蛋白质组（acetylproteome）、泛素化蛋白质组（ubiquitylproteome）、表观基因组（epigenome）与免疫组（immunome）的表征分析。本研究产生的数据集涵盖19种组织、25个分子检测平台，以及年轻成年雌雄大鼠的4个训练时间点，共计9466项检测实验。

数据贡献方：物理活动分子转导子联盟（Molecular Transducers of Physical Activity Consortium, MoTrPAC）