Gene expression profiling of human angiosarcoma. Gene expression profiling of human angiosarcoma

We generated RNA-Seq data from human angiosarcoma tissues (N=13) and non-malignant tissue samples (N=6). The goal of this study was to determine differentially expressed genes in the tumors compared to controls. We sequenced about 80 – 100 million sequence reads per sample and mapped to the human reference genome (GRCh38). We identified 1,237 differentially expressed genes between the tumors and non-malignant controls (FDR P-value < 0.05): 490 genes were upregulated and 747 genes were downregulated in angiosarcomas. Our results show a comprehensive data of fusion genes and gene expression profile in human angiosarcomas using RNA-seq technology. Overall design: Genome-wide gene expression profiling between human angiosarcomas and non-malignant control tissues using RNA-seq.

本研究从13例人血管肉瘤（angiosarcoma）组织样本与6例非恶性组织对照样本中获取了RNA测序（RNA-Seq）数据。本研究的核心目标为鉴定肿瘤样本与对照样本间的差异表达基因。所有样本的测序读段数约为8000万至1亿条，测序数据均比对至人类参考基因组GRCh38。本研究共鉴定出肿瘤与非恶性对照样本间的1237个差异表达基因（错误发现率（FDR）P值<0.05），其中血管肉瘤组织中490个基因呈上调表达，747个基因呈下调表达。本研究通过RNA测序技术，获取了人血管肉瘤中融合基因与基因表达谱的完整数据集。整体实验设计：采用RNA测序技术，对人血管肉瘤与非恶性对照组织进行全基因组基因表达谱分析。