Cumulative in-cis mutagenesis in vivo reveals various functions for CTCF sites at the mouse Hoxd cluster [RNA-seq]

RNA-seq analysis of the effect of CRISPR/Cas9-induced CTCF binding sites deletions on gene expression. We studied E12.5 distal or proximal forelimbs of wildtype and mutant alleles. Overall design: RNA-seq analysis of the effect of CRISPR/Cas9-induced CTCF binding sites deletions on gene expression. We studied E12.5 distal or proximal forelimbs of wildtype and mutant alleles.

本研究通过RNA测序（RNA-seq）分析CRISPR/Cas9（成簇规律间隔短回文重复序列相关蛋白9）诱导的CCCTC结合因子（CTCF）结合位点缺失对基因表达的影响。我们对野生型（wildtype）与突变等位基因（mutant alleles）的E12.5（胚胎发育第12.5天，embryonic day 12.5）胎鼠前肢远端或近端组织开展研究。整体实验设计：通过RNA测序分析CRISPR/Cas9诱导的CCCTC结合因子结合位点缺失对基因表达的影响。我们对野生型与突变等位基因的E12.5胎鼠前肢远端或近端组织开展研究。