Data from: Bud production, dormancy, and mortality patterns differ by growth form and photosynthetic pathway following high-energy fire

Understanding long-term perennial bud bank dynamics will lead to better prediction of graminoid survival and regrowth following high-energy grassland restoration fires and facilitate manager decision-making regarding restoration in woody encroached grasslands. Experimental research to assess long-term bud bank dynamics of two dominant perennial grasses with different growth forms and physiologies, <i>Nassella leucotricha</i> and <i>Hilaria belangeri</i>, in a Texas semi-arid savanna and determine the impact of fire energy on the recovery of their bud bank dynamics. A total of 36 plots, each 10x10m, were demarcated and centered on a mature mesquite shrub within a relatively homogeneous pasture with a uniform management history. Plots were at least 1.8m apart to facilitate burning each plot as an individual unit. Fire treatments, consisting of low-energy, high-energy, and unburned control, were replicated 12 times and applied in a randomized design in late July 2018.Tillers were harvested approximately every two weeks during the growing season and once a month during the dormant season. The buds associated with each tiller were counted and their activity classified as either active, dormant, or dead using the Tetrazolium and Evans Blue staining procedures.

阐明长期多年生芽库动态，可更为精准地预测高强度草原恢复火烧后禾草类植物的存活与再生长情况，同时助力管理者制定木本入侵草原的恢复决策。本实验旨在评估美国德克萨斯州半干旱稀树草原中两种具有不同生长型与生理特性的优势多年生禾草——白绒毛针茅（*Nassella leucotricha*）与贝氏扁芒草（*Hilaria belangeri*）的长期芽库动态，并明确火强度对其芽库动态恢复的影响。
本研究共划定36个面积为10m×10m的样地，每个样地均以管理历史统一的均质牧场内的成熟牧豆树灌丛为中心。样地之间间距不小于1.8m，以确保每个样地可作为独立单元开展火烧作业。本研究设置低强度火、高强度火以及未火烧对照三种火烧处理，每组处理设置12次重复，并于2018年7月末采用完全随机设计实施处理。
在生长季每两周左右采集一次分蘖株，休眠季则每月采集一次。对每株分蘖的芽体进行计数，并通过四唑盐（Tetrazolium）与伊文思蓝（Evans Blue）染色法，将芽体的活性划分为活跃、休眠或死亡三类。