UVB drives transcriptomic and epigenetic reprogramming which can be protected by antioxidant sulforaphane in human skin keratinocytes [Methyl-seq]

Continuous exposure to ultraviolet (UV) is one of the main factors contributing to skin carcinogenesis. Sulforaphane (SFN) is a potent antioxidative agent which has potential to prevent the UV-induced skin cell transformation. We characterized the transcriptome and CpG methylation profile of human keratinocytes (HaCaT cells) treated with UVB and/or SFN using RNA sequencing. Samples of HaCaT cells were used for extraction of DNA and RNA; DNA was enriched by Agilent SureSelect Kit then bisulfite converted methyl-seq was performed. RNA-seq was performed with routine. Strategy. This dataset contains data for the Methyl-seq only; Check the other SubSeries under this SuperSeries for RNA-seq data.

持续暴露于紫外线（ultraviolet, UV）是诱发皮肤癌变的主要因素之一。萝卜硫素（Sulforaphane, SFN）是一种强效抗氧化剂，具备预防紫外线诱导的皮肤细胞转化的潜在能力。本研究采用RNA测序（RNA sequencing）技术，对经UVB及/或SFN处理的人角质形成细胞（human keratinocytes, HaCaT细胞）的转录组（transcriptome）与CpG甲基化谱（CpG methylation profile）进行了系统表征。本实验使用HaCaT细胞样本提取DNA与RNA：DNA通过安捷伦SureSelect试剂盒（Agilent SureSelect Kit）完成富集，随后开展亚硫酸氢盐转化甲基测序（bisulfite converted methyl-seq）。RNA测序（RNA-seq）采用常规实验策略进行。本数据集仅包含甲基测序（Methyl-seq）相关数据；RNA测序数据请查看本超级数据集（SuperSeries）下的其他子数据集（SubSeries）。