Validation of microarray data by qPCR and protein levels.

Mouse bone marrow cells were differentiated in GM-CSF for 6 days in the presence of 200 nM sTM from day 4 to 6. DCs were then analyzed for the expression of cell surface markers by flow cytometry while RNA was analyzed on microarrays and by qPCR. In some experiments protein levels in the medium was determined by ELISA. The direction of change in levels of protein is represented by arrows. CD206 is also known as mannose receptor C type 1 (MRC1). The mRNA data shows the results for both the A and B chains of IL-12p70. ND: not determined; the value of the change in gene expression in qPCR was determined by ratio of ΔΔct. The FACS and ELISA data were taken from Takagi et al [11].

小鼠骨髓细胞在粒细胞-巨噬细胞集落刺激因子（GM-CSF）中诱导分化6天，其中第4天至第6天添加200 nM可溶性血栓调节蛋白（sTM）。随后通过流式细胞术（flow cytometry, FACS）分析树突状细胞（DCs）的细胞表面标志物表达，同时采用基因芯片（microarrays）与定量聚合酶链反应（qPCR）对RNA进行检测分析。部分实验中通过酶联免疫吸附试验（ELISA）测定培养基中的蛋白含量。蛋白水平的变化方向以箭头示意。CD206又称为甘露糖受体C型1（MRC1）。信使核糖核酸（mRNA）数据涵盖白细胞介素-12p70（IL-12p70）的A、B两条链的检测结果。ND：未测定（not determined）；定量PCR中基因表达变化值通过ΔΔct比值计算得出。本研究的流式细胞术（FACS）与ELISA数据取自Takagi等人[11]的研究成果。