Data for: Virulence properties and sensitivity profile of Candida parapsilosis complex species and Kodamaea ohmeri isolates from onychomycosis of HIV/AIDS patients

This study investigated the in vitro adhesion and biofilm formation capacity of Candida parapsilosis and Kodamaea ohmeri isolates from onychomycoses of HIV/AIDS patients and also established the antifungal sensitivity profiles of these isolates. Onychomycosis in HIV/AIDS patients showed a high prevalence of emerging yeasts, among which C. parapsilosis complex species and K. ohmeri were the most frequent. Three C. parapsilosis sensu stricto isolates and two C. orthopsilosis isolates were resistant to amphotericin B and 83% were resistant to terbinafine. All isolates adhered to stainless steel and siliconized latex surfaces, and carbohydrates intensified adhesion of all isolates. Isolates adhered to keratinous nail and 50% formed biofilms with strong intensity. In multispecies or polymicrobial biofilms, C. albicans and Staphylococcus aureus regulated the formation of biofilms, decreasing the number of non-albicans species and of Candida spp. cells, respectively. Nonetheless, the presence of C. parapsilosis sensu stricto or K. ohmeri in mixed biofilms increased the number of viable C. albicans cells. The isolation of emerging yeast species from onychomycosis with high adhesion capacity to medical devices, with many being good biofilm producers, is a result that should be considered relevant in clinical practice. In addition, half of the isolates resistant to at least one of the tested antifungals or were susceptible in a dose-dependent manner, which corroborates the infectious capacity and viability of these isolates.

本研究针对来源于HIV/AIDS患者甲真菌病（onychomycosis）样本的近平滑念珠菌（Candida parapsilosis）及奥默柯达酵母（Kodamaea ohmeri）分离株，开展了体外黏附与生物膜形成能力研究，并明确了上述分离株的抗真菌药敏谱。HIV/AIDS患者的甲真菌病中，新兴酵母菌检出率较高，其中近平滑念珠菌复合群物种及奥默柯达酵母为最常见的分离菌。3株近平滑念珠菌严格型（Candida parapsilosis sensu stricto）分离株与2株奥氏假丝酵母菌（Candida orthopsilosis）分离株对两性霉素B（amphotericin B）耐药，且83%的分离株对特比萘芬（terbinafine）耐药。所有分离株均可黏附于不锈钢及硅化乳胶表面，碳水化合物可增强所有分离株的黏附能力。分离株可黏附于角质化指甲表面，其中50%的分离株可形成强度较强的生物膜。在多菌种或多微生物生物膜中，白念珠菌（Candida albicans）与金黄色葡萄球菌（Staphylococcus aureus）分别可调控生物膜形成，分别减少非白念珠菌属物种及念珠菌属（Candida spp.）细胞的数量。尽管如此，混合生物膜中近平滑念珠菌严格型或奥默柯达酵母的存在，可提高存活白念珠菌的细胞数量。从甲真菌病样本中分离出的新兴酵母菌，对医疗器械具有较高黏附能力，且多数可形成优质生物膜，该研究结果在临床实践中具有重要参考价值。此外，半数分离株对至少一种受试抗真菌药物耐药，或呈现剂量依赖性敏感，该结果佐证了上述分离株的感染能力与存活能力。