Genome-wide analysis of gene expression in MLL translocation transformed mouse leukemia cell lines. Mus musculus

The purpose of this study is to investigate the transcriptional programs as it relates to disease latency initiated by different MLL fusion proteins, including: MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 and MLL-ENL. Leukemia cell lines were established by transforming kit+ mouse bone marrow cells with retroviruses coding MLL-AF1p, MLL-AF6, MLL-Gas7, MLL-AF9 or MLL-ENL. At early phase after the cell lines were established, cells growing at exponential phase (cell density at 0.5~1x106/ml) were harvested for RNA extraction and sequencing purpose. Overall design: Sequencing is performed on total RNA isolated from mouse leukemia cell lines generated from kit+ mouse bone marrow cells transduced with various MLL fusion proteins and is compared to control total RNA isolated from kit+ mouse bone marrow cells.

本研究旨在探究不同混合谱系白血病（MLL）融合蛋白引发的疾病潜伏期相关转录程序，涉及的融合蛋白包括MLL-AF1p、MLL-AF6、MLL-Gas7、MLL-AF9及MLL-ENL。研究人员通过编码上述MLL融合蛋白的逆转录病毒转化kit+小鼠骨髓细胞，构建白血病细胞系；在细胞系构建初期，收取处于指数生长期（细胞密度为0.5~1×10^6/ml）的细胞，用于RNA提取与测序实验。实验设计：对经不同MLL融合蛋白转导的kit+小鼠骨髓细胞所构建的小鼠白血病细胞系中提取的总RNA进行测序，并与取自kit+小鼠骨髓细胞的对照总RNA进行比对分析。