CIL:45896, Homo sapiens. In Cell Image Library

Plate 25674 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号25674的检测板——表型谱分析（phenotypic profiling）旨在通过对每个样本的细胞表型开展多参数、基于特征的系统性分析，实现样本特征的凝练，使得不同样本的谱间相似性能够反映样本本身的真实相似性。本图像集为测试基于图像的谱分析方法（image-based profiling methods）区分小分子化合物作用效果的能力提供了基准数据集。

图像数据集
本数据集的图像来自经1600种已知生物活性化合物分别处理的U2OS细胞，样本经六种标记物染色以表征七种细胞器的分布，该实验为细胞染色成像（cell-painting）实验。

本次预实验共包含20块检测板。每块检测板设有384个培养孔，每个孔采集9个成像视野，总计69120个成像视野。每个视野通过5个成像通道（即检测波长）进行成像，每个通道的图像均以独立的灰度图像文件存储，因此本数据集共包含345600个16位TIFF格式的图像文件。