Microsatellite genotypes and partial COI fragments for 5 cod species in the Chukchi Sea, Arctic Integrated Ecosystem Research Program, 2019

These data are part of the Arctic IES component of the Arctic Integrated Ecosystem Research Program (IERP), sponsored by the North Pacific Research Board (NPRB), Collaborative Alaskan Arctic Studies Program (formerly the North Slope Borough/Shell Baseline Studies Program), Bureau of Ocean Energy Management (BOEM), and the Office of Naval Research Marine Mammals and Biology Program. Initially, Arctic Cod (Boreogadus saida) was collected from the Chukchi Sea for genetic population structure analyses. Bulk samples of approximately 50 fish were retained from each midwater trawls. Cod samples were collected in August and September, 2019 for species identification. These samples were examined at 15 microsatellite loci and genotypes were recorded in an excel spreadsheet (two fragment sizes for each specimen). Additionally, phase 2 of the project was initiatiated, because the microsatellite analysis detected other cod species in the Arctic Cod sample. Fin clips from other cod projects were collected for genetic analysis to verify species identification (n=1545). mtDNA was examined at a partial (100 base pair) COI fragment and aligned with known sequences from other possible cods. Sequences are stored in a text file.

本数据集隶属于北极综合生态系统研究计划（Arctic Integrated Ecosystem Research Program，缩写IERP）的北极IES子项目，由北太平洋研究委员会（North Pacific Research Board，缩写NPRB）、阿拉斯加北极合作研究计划（前身为北坡自治市镇/壳牌基线研究计划）、海洋能源管理局（Bureau of Ocean Energy Management，缩写BOEM）以及美国海军研究办公室海洋哺乳动物与生物学项目资助。

最初，研究人员从楚科奇海采集北极鳕鱼（*Boreogadus saida*）用于群体遗传结构分析。每次中层拖网作业留存约50尾鱼作为混合样本。2019年8月至9月，研究人员采集鳕鱼样本以开展物种鉴定工作。

对上述样本的15个微卫星位点（microsatellite loci）进行检测，并将基因型信息记录于Excel电子表格中（每份样本对应两个片段长度）。由于微卫星分析在北极鳕鱼样本中检出了其他鳕鱼物种，项目第二阶段随即启动。

研究人员采集了其他鳕鱼研究项目的鳍剪样本，用于遗传分析以验证物种鉴定结果，有效样本量为1545份（n=1545）。对线粒体DNA（mtDNA）的一段100碱基对的COI片段进行检测，并与其他潜在鳕鱼物种的已知序列进行比对。所有序列均存储于文本文件中。