The dual roles of the TLR2/TLR4/NLRP3-H-PGDS-PGD2 axis in regulating the inflammatory response in E. coli-induced bovine bone marrow-derived macrophages and endometrial tissue

Antibiotic treatment for endometritis in dairy cows demonstrates some effectiveness; however, due to insufficient consideration of the underlying pathogenesis, these treatments do not significantly enhance cure rates or improve subsequent fertility in affected cows. Therefore, we focus on the pathogenesis of bovine endometrial diseases and the role of prostaglandin compounds in endometrial inflammation. In this study, E. coli-infected bovine bone marrow-derived macrophages were used as a model for endometritis in dairy cows. We successfully isolated and characterized these macrophages and screened drug concentrations for treating endometritis, providing valuable evidence for developing novel veterinary therapies to improve endometritis's cure rate in dairy cows.

奶牛子宫内膜炎的抗生素治疗虽具备一定疗效，但因未充分考量其潜在发病机制，此类治疗无法显著提升患病奶牛的治愈率，亦无法改善其后续生育能力。因此，本研究聚焦于牛子宫内膜疾病的发病机制，以及前列腺素类化合物在子宫内膜炎症中的作用。本研究以大肠杆菌（E. coli）感染的牛骨髓源巨噬细胞作为奶牛子宫内膜炎的体外模型，成功分离并鉴定了此类巨噬细胞，同时筛选出可用于治疗子宫内膜炎的药物浓度，为开发新型兽药疗法以提升奶牛子宫内膜炎的治愈率提供了极具价值的实验依据。