Transcriptome analysis of a novel alveolar porcine epithelial cells line infected with different live attenuated Influenza Viruses. Transcriptome analysis of a novel alveolar porcine epithelial cells line infected with different live attenuated Influenza Viruses

The project aim at studying transcriptional changes by BRB-seq of porcine alveolar epithelial cells line infected with wild type influenza virus compared to live attenuated influenza viruses. In addition, primary porcine nasal epithelial cells was also added to be able to compare how the alveolare epithelial cells line compare to primary cells. Finally, additional positive control including procine alveolar epithelial cells stimulated with Poly I:C or infected with IFN inducing Sendei virus was added.

本项目旨在通过BRB-seq技术，研究感染野生型流感病毒与减毒活流感病毒的猪肺泡上皮细胞系的转录组变化。此外，本研究还纳入了猪原代鼻上皮细胞，以对比该肺泡上皮细胞系与原代细胞的差异。最后，本研究增设了额外的阳性对照：包括经聚肌胞苷酸（Poly I:C）刺激，或感染诱导干扰素的仙台病毒（Sendai virus）的猪肺泡上皮细胞。