Transcriptional profiling of Regulatory T cells isolated from mice exposed to Smoke, Morphine and Smoke plus Morphine

Purpose: The goals of this study were to identify gene expression signatures that are unique to Tregs from mice upon treatments with smoke, morphine and smoke plus morphine Methods and results: Wild-type mice were treated with cigarette smoke (SM) for a period of eight weeks, and/or the chronic continuous administration of morphine (M) via mini-pumps for the final four weeks CD4+CD25highCD127low Tregs from spleen were purified by cell sorting to generate mRNA transcription. Transcriptional profiling revealed a unique Treg signatures in SM, M or SM + M relative to control mice. Conclusion: Our study represents the first detailed analysis of splenic Treg transcriptome in mice exposure to smoke plus morphine. Spleen treg mRNA profiles of mice treated with smoke, morphine, or smoke plus morphine.

研究目的：本研究旨在明确经香烟烟雾、吗啡以及香烟烟雾联合吗啡处理后，小鼠调节性T细胞（Tregs）所特有的基因表达特征。
方法与结果：将野生型小鼠暴露于香烟烟雾（SM）中8周，并在最后4周通过微型渗透压泵（mini-pumps）慢性持续给予吗啡（M），或联合两种处理方式。通过细胞分选纯化脾脏来源的CD4+CD25highCD127low调节性T细胞（Tregs）以提取mRNA进行转录组分析。转录谱分析（Transcriptional profiling）结果显示，相较于对照组小鼠，香烟烟雾组、吗啡组以及烟雾联合吗啡组小鼠的脾脏调节性T细胞均呈现出独特的特征性基因表达谱。
结论：本研究首次对暴露于香烟烟雾联合吗啡的小鼠脾脏调节性T细胞转录组进行了详细分析。本数据集涵盖经香烟烟雾、吗啡或香烟烟雾联合吗啡处理的小鼠脾脏调节性T细胞的mRNA表达谱数据。