Lazrak-dataset

Electrophysiology data of ENaC activity in human and mouse alveolar epithelial cells in-situ, in freshly cut lung slices, and in culture using patch clamp techniques and Ussing chamber system for measuring short circuit currents across alveolar cells confluents monolayers. In addition, the project will generate lung tissue images for the distribution and the expression of the calcium sensing receptor in the alveolar epithelium. The expression of ENaC and CaSR proteins will be measured by western blot producing more data to help clarify the mechanism by which hyaluronan fragments activate calcium-sensing receptor and inhibits ions and water transport across the alveolar epithelium causing lung edema. The collected data from the experiments mentioned above will approximatively total 20 to 30 Gigabytes. The following data files will be produced in the course of the project: Axon binary files (ABF files) when measuring ENaC activity in alveolar cells in-situ, in lung slices. These files are usually larges, tens of megabytes each, depending on the sampling rate and the time necessary to collect enough data and test compounds of interest. In addition, the data analysis will produce new files, axon text files (ATF files), and finally the processed data will be transferred to a third-party analysis and presentation programs ( IGOR from Wavemetrics and Origin from Origin labs) that generate the final files suitable for presentation and publication. The imaging files are very large and remain at the same size after analysis. Western blot files and in-vivo data will be presented as an aggregate and do not generate large files excel and PowerPoint will be used to pool the numbers together and put in a presentable form, respectively.

本数据集涵盖采用膜片钳技术（Patch Clamp）与尤斯灌流室系统（Ussing Chamber System）获取的电生理数据，分别针对原位状态、新鲜离体肺切片内及体外培养的人源与小鼠肺泡上皮细胞的上皮钠通道（ENaC，Epithelial Sodium Channel）活性；其中尤斯灌流室系统用于检测肺泡上皮细胞汇合单层的跨上皮短路电流。此外，本项目还将生成肺组织成像数据，用于分析钙敏感受体（CaSR，Calcium-Sensing Receptor）在肺泡上皮中的分布与表达情况。本项目将通过蛋白质免疫印迹（Western Blot，WB）检测上皮钠通道与钙敏感受体的蛋白表达水平，相关实验将产生额外数据，以阐明透明质酸片段激活钙敏感受体、抑制肺泡上皮跨离子与水转运并进而引发肺水肿的具体机制。上述所有实验采集的数据总量约为20至30吉字节（GB）。本项目将生成以下类型的数据文件：针对原位及肺切片中肺泡上皮细胞的上皮钠通道活性检测环节，将生成Axon二进制文件（ABF files）；此类文件单个体积通常较大，可达数十兆字节，具体大小取决于采样率与采集足量数据及测试目标受试化合物所需的时长。此外，数据分析环节将生成Axon文本文件（ATF files）；最终处理完成的数据将导入第三方分析与绘图软件——Wavemetrics公司的IGOR Pro及OriginLabs公司的Origin——以生成适用于汇报与发表的最终文件。成像文件体积普遍较大，且经分析后文件大小无明显变化。蛋白质免疫印迹实验数据与体内实验数据将以汇总形式呈现，无需生成大体积文件；相关数据将分别通过Excel汇总数值、PowerPoint制作可展示格式的汇报材料。