miRNA transcriptome profiling of Chlorella sorokiniana and Chlorella zofingiensis under normal and stress condition

Purpose: The purpose of this study are to identify the miRNA involved in regulating the production of metabolites in Chlorella sorokiniana and Chlorella zofingiensis under normal and stress-induced condition through RNA-sequencing technique. Methods: miRNA transcriptome profile from normal and stress sample of C. sorokiniana and C. zofingiensis were generated, in triplicate, using Illumina Miseq. The sequence reads that passed quality filters were analysed using CLC genomic workbench and OmiRas. Results: The known and predicted novel miRNAs were identified. Although most of the identified miRNAs were not functionally determined, this study suggests that they were species-specific, which may have roles in regulating genes during stress related condition. Overall design: miRNA transcriptome profile of C. sorokiniana and C. zofingiensis under normal and stress conditions were generated in triplicates by deep sequencing using NEBNext Multiplex Small RNA Library Prep for Illumina.

研究目的：本研究旨在通过RNA测序（RNA-sequencing）技术，鉴定正常及胁迫诱导条件下，参与调控索氏小球藻（Chlorella sorokiniana）和佐夫奇小球藻（Chlorella zofingiensis）代谢物生成的微小RNA（microRNA，miRNA）。
实验方法：采用Illumina Miseq平台，对索氏小球藻（C. sorokiniana）和佐夫奇小球藻（C. zofingiensis）的正常样本与胁迫样本的miRNA转录组进行三次生物学重复测序。对通过质量过滤的序列读段，将采用CLC基因组工作台（CLC genomic workbench）与OmiRas工具进行分析。
实验结果：本研究鉴定出了已知的微小RNA及预测得到的新型微小RNA。尽管多数已鉴定的微小RNA尚未明确其功能，但本研究表明它们具有物种特异性，可能在胁迫相关条件下参与基因表达调控。
整体实验设计：采用适配Illumina测序平台的NEBNext多重小RNA文库制备试剂盒，通过深度测序，对正常及胁迫条件下的索氏小球藻（C. sorokiniana）和佐夫奇小球藻（C. zofingiensis）的miRNA转录组进行三次生物学重复测序，生成对应的转录组图谱。