Expression data from APC 50% or 80% -treated HFDPCs. Expression data from APC 50% or 80% -treated HFDPCs

Gene expression profiling reveals a potential role of APC 50% or APC 80% in stimulating hair growth in dermal papilla cells. HFDPCs were human primary cells line, treated with 5 μg/ml APC 50% for 48 h. Microarray gene expression profiling was conducted for three biological replicates HFDPCs were human primary cells line, treated with 5 μg/ml APC 80% for 48 h. Microarray gene expression profiling was conducted for three biological replicates Overall design: RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). Two hundred and fifty ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.

基因表达谱分析揭示了APC 50%与APC 80%在真皮乳头细胞中促进毛发生长的潜在作用。
实验分组如下：人类原代细胞系HFDPCs经5 μg/ml的APC 50%处理48小时后，设置3次生物学重复以开展芯片基因表达谱分析；另一组人类原代细胞系HFDPCs经5 μg/ml的APC 80%处理48小时后，同样设置3次生物学重复以开展芯片基因表达谱分析。

实验整体设计：
总RNA提取采用Isogen试剂（日本富山市日本基因株式会社）；RNA完整性通过NanoDrop 2000分光光度计（美国特拉华州威尔明顿赛默飞世尔科技公司）进行定量检测。
采用GeneChip® 3'表达芯片及3'体外转录（IVT）PLUS试剂盒（美国加利福尼亚州圣克拉拉市Affymetrix公司）制备用于基因表达谱分析的RNA样本。按照操作手册，每份样本取250 ng总RNA，从总RNA中的聚腺苷酸（poly(A)）RNA扩增并制备生物素标记的互补RNA（cRNA），体外转录孵育时长为16小时。
采用GeneAtlas®杂交、洗涤与染色试剂盒（产品编号P/N 08-0306），按照操作手册对3' IVT芯片条带进行杂交。人类基因组芯片条带（HG-U219）于45℃恒温箱中杂交16小时，随后完成洗涤与染色，最终通过GeneAtlas流体力学与成像工作站完成芯片扫描成像。