Characterization of the Forkhead transcription factor fhpA in Aspergillus flavus

The fhpA gene was disrupted in a CA14 Aspergillus flavus stain utilizing the Aspergillus oryzae pyrithimaine (ptrA) selectable marker. Genomic DNA (gDNA) was isolated from this strain utilizing the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, D6005). Isolated gDNA was sent to Novogene Corporation (Sacramento, CA, USA) for whole genome sequencing. Genomic DNA libraries were constructed and quantified using standard Novogene protocols. Quantified libraries were pooled and sequenced on an Illumina NovaSeq 6000 sequencer.

本研究采用米曲霉（Aspergillus oryzae）的嘧啶霉素（pyrithimaine）抗性选择标记ptrA，对CA14黄曲霉（Aspergillus flavus）菌株中的fhpA基因实施了敲除。采用Quick-DNA真菌/细菌微量基因组DNA提取试剂盒（Zymo Research，货号D6005）从该菌株中分离得到基因组DNA（gDNA）。将提取获得的gDNA送至位于美国加利福尼亚州萨克拉门托的诺禾致源公司（Novogene Corporation）开展全基因组测序。按照诺禾致源的标准实验流程完成基因组DNA文库的构建与定量。将定量合格的文库进行混合，随后在Illumina NovaSeq 6000测序仪上完成测序。