Linking the Belowground Microbial Composition, Diversity and Activity to Soilborne Disease Suppression and Growth Promotion of Tomato Amended with Biochar

This study was designed to establish the relationship between biochar-induced changes in rhizosphere microbial community structure, taxon-functional diversity, and microbial activity with soilborne disease suppression and enhanced plant performance in a comprehensive fashion. We sampled four independent replicates soil samples. To determine the effect of biochar treatments on the diversity and composition of root associated bacterial communities we applied a high-throughput sequencing-based protocol that targets PCR-generated amplicons from the variable regions V4 of the 16S rRNA gene using the bacterial primer set 515F/806R. Fastq files of 8 libraries were produced as a result of Illumina MiSeq technology at the Research Resources Center of the University of Illinois at Chicago.

本研究旨在全面明确生物炭诱导的根际微生物群落结构、类群功能多样性及微生物活性变化，与土传病害抑制及植物生长提升之间的关联。我们采集了4组独立重复的土壤样品。为探究生物炭处理对根系相关细菌群落多样性与组成的影响，本研究采用基于高通量测序的实验方案：以细菌引物对515F/806R靶向扩增16S核糖体RNA（16S rRNA）基因V4可变区的PCR产物作为测序靶点。本研究于伊利诺伊大学芝加哥分校研究资源中心使用Illumina MiSeq测序平台完成测序，最终得到8个文库的Fastq格式测序文件。