PolyA-sequencing in Jurkat cells upon CDK12 depletion.

We analyzed the 3' end of polyadenylated transcripts in Jurkat cells treated with either THZ531 (vs. DMSO) or the CDK12 degrader BSJ-4-116 (vs. BSJ-4-116NC). Overall design: Expression of polyA transcripts in Jurkat cells treated with 250 nM THZ531 (vs. DMSO control) or 50 nM BSJ-4-116 (vs. BSJ-4-116NC) for 8h using the RNA-seq library kit (QuantSeq 3' mRNA Sequencing REV, Lexogen) in triplicate, for a total of 12 individual samples.

本研究对经THZ531（相较于DMSO对照）或CDK12降解剂BSJ-4-116（相较于BSJ-4-116NC）处理的Jurkat细胞（Jurkat cells）中多聚腺苷酸化转录本（polyadenylated transcripts）的3'端进行了分析。
实验设计概述：以250 nM THZ531处理Jurkat细胞，处理时长为8小时，以DMSO作为对照；或以50 nM BSJ-4-116处理Jurkat细胞，处理时长同样为8小时，以BSJ-4-116NC作为对照。实验采用Lexogen公司的QuantSeq 3' mRNA Sequencing REV RNA测序建库试剂盒进行建库，每个实验分组均设置3次生物学重复，共计12个独立样本。