Remote ischemic conditioning protects against testicular ischemia∕reperfusion injury in rats

Abstract Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemia∕reperfusion (I∕R) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows: Sham, I∕R, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mg∕kg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p<0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.

摘要
研究目的：评估联合应用N-乙酰半胱氨酸（N-acetylcysteine，简称NAC）的远程缺血预处理对大鼠睾丸缺血再灌注（ischemia/reperfusion，I/R）损伤的保护效果。
方法：将25只成年雄性Wistar大鼠随机分为5个实验组（每组n=5），具体分组如下：假手术组（Sham）、缺血再灌注组（I/R）、缺血预处理组（PER）、NAC组以及PER+NAC联合组。通过将左侧睾丸顺时针旋转720°构建2小时缺血模型，随后实施4小时再灌注。缺血预处理于再灌注前30分钟进行，通过对左侧肢体实施3次、每次10分钟的I/R循环完成。N-乙酰半胱氨酸（150 mg/kg）于再灌注前30分钟给予。
结果：血浆丙二醛（malondialdehyde，MDA）水平方面，缺血再灌注组与其余各组均存在显著统计学差异（p<0.01）；此外，缺血预处理组与假手术组、PER+NAC联合组之间的血浆MDA水平亦存在统计学差异（p<0.05）。
结论：本研究证实，单独应用缺血预处理可减轻氧化应激相关的脂质过氧化反应，发挥睾丸保护作用。然而，当缺血预处理与N-乙酰半胱氨酸联合使用时，并未表现出对抗睾丸缺血再灌注损伤的保护效果。