Additional file 2: of Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes

Figure S1. Effect of siRNAs or DNA expression plasmids transfections in C-MSCs. Figure S2. MSCs cultured on 2D plastic culture plate maintained YAP/TAZ activity. Figure S3. High YAP/TAZ activity in subconfluent MSCs cultured on a 2D plastic plate regulates the cell lineage into osteogenesis but not adipo/chondrogenesis. Figure S4. Disruption of F-actin integrity by ROCK inhibitor and acto-myosin inhibitor abrogates the TAZS89A-induced positive feedback loop for in C-MSCs. (ZIP 6647Â kb)

补充图S1：小干扰核糖核酸（siRNAs）或DNA表达质粒转染对C-MSCs的影响。补充图S2：在二维塑料培养板中培养的间充质干细胞（MSCs）可维持YAP/TAZ活性。补充图S3：二维塑料培养板上处于亚汇合状态的MSCs中高活性的YAP/TAZ可调控细胞向成骨细胞谱系分化，而非脂肪/软骨细胞谱系分化。补充图S4：ROCK抑制剂与肌动蛋白-肌球蛋白抑制剂破坏F-肌动蛋白（F-actin）的完整性后，可阻断C-MSCs中TAZS89A诱导的正反馈环路。（ZIP 6647 KB）