The effect of 2(5H)-furanone on Pseudoaltermonas marina biofilm formation and the settlement of the mussel Mytilus coruscus

Bacterial quorum sensing (QS) is a chemical communication that allows bacteria to coordinate their population and gene regulation (e.g. development of biofilm, secretion of secondary metabolites) in response to desirable concentrations of QS signals. Here, we studied the effect of QS inhibitor 2(5H)-furanone on the formation of Pseudoaltermonas marina biofilms, and the effect of these biofilms on the settlement of the mussel Mytilus coruscus. 2(5H)-furanone was added during the biofilms formation of P. marina or in the plantigrades settlement bioassay. The adding of 2(5H)-furanone (10-4M) during biofilms formation significantly (p < 0.05) reduced the settlement of M. coruscus plantigrades. In order to understand the differences of underlying molecular mechanisms between the PC group and furanone treated group during biofilm formation, 10-4 M FUR treated BFs were selected for transcriptome sequencing due to lowest settlement.

细菌群体感应（Quorum Sensing, QS）是一类化学通讯机制，可使细菌在群体感应信号分子浓度达到适宜水平时，协同调控种群密度与基因表达，例如生物被膜形成、次级代谢产物分泌。本研究探究了群体感应抑制剂2(5H)-呋喃酮对海假交替单胞菌（Pseudoaltermonas marina）生物被膜形成的影响，以及该菌生物被膜对厚壳贻贝（Mytilus coruscus）匍匐稚贝附着的调控作用。实验分别在海假交替单胞菌生物被膜形成阶段，以及厚壳贻贝匍匐稚贝附着生物测定过程中添加2(5H)-呋喃酮。在生物被膜形成阶段添加10⁻⁴ M的2(5H)-呋喃酮，可显著降低厚壳贻贝匍匐稚贝的附着率（p < 0.05）。为解析PC组与呋喃酮处理组在生物被膜形成过程中的潜在分子机制差异，本研究选取附着率最低的10⁻⁴ M FUR处理的生物被膜样本开展转录组测序。