Transcriptional profiling of Schistosoma mansoni following knocking down Krüppel-like factor 4 (klf4) [RNA-Seq]

klf4 is enriched in a putative tegument associated lineage of S. mansoni. To investigate the function of klf4, we compared transcriptional profiles of klf4 (RNAi) worms to those of control (RNAi) worms. Overall design: Adult male parasites were treated with either klf4-targeting dsRNA or control dsRNA for 17 days, then subjected to RNAseq.

klf4（Krüppel样因子4）在曼氏血吸虫（Schistosoma mansoni）的推定体被相关谱系中富集。为探究klf4的功能，本研究对比了经klf4靶向RNA干扰（RNA interference, RNAi）处理的虫体与对照RNA干扰处理的虫体的转录组谱。实验设计：将成年雄性寄生虫用靶向klf4的双链RNA（double-stranded RNA, dsRNA）或对照双链RNA处理17天，随后进行RNA测序（RNA sequencing, RNAseq）。