Maize transcriptomic responses to Pantoea stewartii vascular infection in B73 and pan1 (R1) lines (experiment A).

To gain insight into the genetic and molecular mechanisms that underlie the enhanced resistance of pan1 lines to vascular spread of P. stewartii, we used RNA-seq to compare the transcriptomes of B73 and pan1 lines one day after mock or bacterial inoculation of leaf veins, being this the first analysis of maize transcriptional responses to P. stewartii infection. Overall design: 24 samples were analyzed (3 genotypes x 2 treatments x 4 replicates). Genotyopes: B73, pan1-ems/pan1-ems (R1) and pan1-ems/+ (R1/B73). Treatments: P. stewartii-GFP infection and Mock inoculation (serving as wounding control). Four biological replicates were analyzed.

为解析pan1株系对斯氏假单胞菌（P. stewartii）维管扩散增强抗性的遗传与分子机制，本研究借助RNA测序（RNA-seq）对比了B73与pan1株系在叶脉经模拟接种或细菌接种1天后的转录组；这亦是首个针对玉米受斯氏假单胞菌感染后的转录响应开展的分析。
实验整体设计：共分析24份样本（3个基因型×2种处理×4次生物学重复）。
基因型包括：B73、pan1-ems/pan1-ems（R1）以及pan1-ems/+（R1/B73）。
处理方式为：斯氏假单胞菌-绿色荧光蛋白（GFP）感染接种与模拟接种（Mock）（作为伤口对照）。
本次分析共设置4次生物学重复。