Regulation of human skin pigmentation in situ by repetitive UV exposure - Molecular characterization of responses to UVA, UVB and SSR. Homo sapiens

To test the hypothesis that different mechanisms and/or factors might be involved in physiological pigmentary responses of the skin to different types of UV, we used whole human genome microarrays and immunohistochemical analyses to characterize human skin in situ to examine how melanocyte-specific proteins and paracrine melanogenic factors are regulated by repetitive exposure to suberythemal doses of different types of UV (UVA, UVB or SSR). Overall design: Six volunteers with skin type II-III were irradiated with SSR, UVA or UVB radiation for 2 weeks (5 times per week, 10 times total) after preliminary determination of their MEDs. Biopsies were taken 3 days after the last irradiation.

为验证"皮肤针对不同类型紫外线的生理性色素反应或涉及不同机制与/或因素"这一假说，本研究采用全人类基因组微阵列（whole human genome microarrays）与免疫组化分析技术，对原位人体皮肤进行表征，以探究反复暴露于不同类型紫外线（UVA、UVB或SSR）的亚红斑剂量下，黑素细胞特异性蛋白及旁分泌黑素生成因子的调控模式。实验设计：共招募6名皮肤类型为II-III型的志愿者，在预先测定其最小红斑剂量（MED, Minimum Erythema Dose）后，分别接受SSR、UVA或UVB照射，每周照射5次，持续2周（总照射次数共计10次）。末次照射3天后采集皮肤活检样本。