Comparison of genome-wide and gene-specific DNA methylation between ART and naturally conceived pregnancies

Data linking assisted reproductive technologies (ART) with aberrant DNA methylation is limited and inconclusive. In addition, most studies to date have analyzed only a small number of CpG sites and focused on methylation changes in placentas, while data on cord blood are scarce. Our aim was to compare DNA methylation in cord blood samples from ART (N = 10) and control pregnancies (N = 8) using a genome-wide approach with the Illumina® Infinium Human Methylation27 array, which interrogates 27,578 CpG sites. A total of 733 (2.7%) of the CpG sites were significantly differentially methylated between the 2 groups (P P

辅助生殖技术（assisted reproductive technologies, ART）与DNA甲基化异常相关的研究数据尚且有限且结论尚不明确。此外，迄今为止的多数研究仅分析了少量CpG位点，且仅聚焦于胎盘组织的甲基化变化，脐血相关数据则十分匮乏。本研究旨在采用全基因组检测策略，借助Illumina® Infinium Human Methylation27芯片（该芯片可检测27578个CpG位点），对比辅助生殖技术受孕组（N=10）与对照组妊娠样本（N=8）的脐血样本DNA甲基化水平。两组间共有733个（占比2.7%）CpG位点存在显著的甲基化差异（P