Expression data from peritoneal macrophages stimulated with lipid A

Setdb1 is one of the H3K9 methyltransferases and represses gene expression by H3K9 methylation. In an attempt to elucidate the role of Setdb1 in the TLR4-mediated inflammatory responses, we performed DNA microarray analysis using lipid A (the active component of LPS)-stimulated peritoneal macrophages from macrophage specific Setdb1 KO (KO) and WT mice. The genes upregulated by lipid A treatment in WT macrophages and further increased in KO macrophages contain many genes associated with interleukins and chemokines. Peritoneal macrophages from WT and KO mice were stimulated with lipid A 10 ng/ml or vehicle for 4 h. Microarray analysis was performed using Affymetrix Mouse 430 2.0.

Setdb1是组蛋白H3赖氨酸9（H3K9）甲基转移酶家族成员之一，可通过催化H3K9位点甲基化抑制基因表达。为阐明Setdb1在Toll样受体4（TLR4）介导的炎症应答中的调控作用，本研究以巨噬细胞特异性Setdb1敲除（KO）及野生型（WT）小鼠的腹腔巨噬细胞为实验对象：将两类细胞分别以10 ng/ml脂质A（lipid A，脂多糖LPS的活性功能片段）或溶剂处理4小时，随后采用Affymetrix Mouse 430 2.0芯片开展DNA微阵列（DNA microarray）分析。分析结果显示，在野生型巨噬细胞中经脂质A处理后表达上调，且在敲除型巨噬细胞中表达进一步升高的基因，大多与白细胞介素及趋化因子家族密切相关。