PCR Amplification and Phylogenetic Analysis of groESL Operon Sequences from Ehrlichia ewingii and Ehrlichia muris

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

本研究采用广谱聚合酶链反应（PCR）引物，扩增了犬类病原体犬埃立克体（Ehrlichia ewingii，近期被确认为人类病原体）以及鼠类病原体鼠埃立克体（Ehrlichia muris）的groESL操纵子部分序列。系统发育分析验证了此前通过16S核糖体RNA（16S rRNA）基因序列比对所确定的埃立克体属物种间演化关系。上述序列为基因序列数据相对匮乏的埃立克体物种提供了额外的PCR扩增靶点。