Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies

ABSTRACT Objective To evaluate the performance of enzyme-linked immunosorbent assay and indirect immunofluorescence methods for the detection of antineutrophil cytoplasmic antibodies in a routine clinical laboratory setting. Methods A total of 227 samples were tested by indirect immunofluorescence and enzyme-linked immunosorbent assay with antigen specificity for antiproteinase 3 and antimyeloperoxidase. The proportions of positive samples were compared by McNemar hypotheses and agreement was described by Cohen’s Kappa coefficient. Results The agreement of the tests was 96.5%, and the Kappa coefficient obtained was 0.70 (95%CI: 0.50-0.90; p

摘要
研究目的：评价常规临床实验室环境下，酶联免疫吸附试验（enzyme-linked immunosorbent assay）与间接免疫荧光法（indirect immunofluorescence）检测抗中性粒细胞胞浆抗体（antineutrophil cytoplasmic antibodies）的应用性能。
方法：共纳入227份样本，分别采用间接免疫荧光法，以及针对抗蛋白酶3（antiproteinase 3）和抗髓过氧化物酶（antimyeloperoxidase）的抗原特异性酶联免疫吸附试验进行检测。采用麦克尼玛假设检验（McNemar hypotheses）比较阳性样本占比，以科恩Kappa系数（Cohen’s Kappa coefficient）评价两种方法的检测一致性。
结果：两种检测方法的一致率为96.5%，所得Kappa系数为0.70（95%置信区间：0.50~0.90；p