Antigens identified for cutaneous lymphoma by proteome serology.

The antigens are listed with reference to the antigen spot numbers as indicated in Figures 2 and 4, their masses and isoelectric points and the outcomes of the peptide mass fingerprints analyses.
*The spot numbers refer to the numbering given for the proteome-serological analyses for the heterogeneous and autologous combinations of tumor cells and sera, respectively.
†The numbers of peaks detected, numbers of peaks assigned and sequence coverage are the results of the peptide mass fingerprint analyses by MASCOT. In case of TIP47 a number of calculated fragments are di-, tri- or tetrapeptides with masses below 500 Dalton which set as threshold in this case.
‡The number of antigen spots refers to all analyses reported herein. Numbers higher than 1 indicate that the same antigen was identified repeatedly in independent protein spots, some in independent electrophoresis gels, vimentin repeatedly in independent 2 gels.

本研究按图2与图4中标注的抗原斑点编号，结合各抗原的相对分子质量、等电点，以及肽质量指纹图谱（peptide mass fingerprints）分析结果，对各类抗原进行列示。
* 本处斑点编号分别对应肿瘤细胞与血清的异源组合、自体组合的蛋白质组血清学分析所采用的编号规则。
† 检测峰数、匹配峰数及序列覆盖度，均为通过MASCOT完成肽质量指纹图谱分析的结果。针对TIP47，本次分析将相对分子质量低于500道尔顿的二肽、三肽及四肽片段设为阈值，此类计算得到的片段均纳入统计范畴。
‡ 本文提及的抗原斑点总数涵盖本研究报告的所有分析结果。编号大于1的情况表示同一抗原在独立的蛋白质斑点中被重复鉴定，部分斑点来自不同的电泳凝胶；其中波形蛋白（vimentin）在两块独立的电泳凝胶中均被重复鉴定到。