Transcriptional network controlled by Candida glabrata Ada2, a transcription coactivator

In order to identify genes governed by Ada2, a transcription coactivator in C. glabrata, we compared the genome-wide expression profile in wild-type strain CBS138 and ada2 mutant via RNA sequencing. According to our analyses, 43 genes were down- and 443 genes were up-regulated in ada2 mutant when compared with the wild-type. The 43 down-regulated genes involved in ergosterol biosynthesis pathway (ERG6, ERG13), adhesin or adhesin-like genes (ICS2, AWP2, EPA7), heat shock genes (HSP30, SSB2) and other cellular functions. Among the 443 up-regulated genes, nine adhesin-like EPA family genes (EPA1, EPA6, EPA8, EPA10, EPA11, EPA12, EPA15, EPA20, EPA23) and three yapsin family genes (YPS4, YPS8, YPS10) were included. Total mRNAs extracted from C. glabrata wild-type CBS138 and ada2 mutant were analyzed using RNA sequencing

为鉴定光滑念珠菌（Candida glabrata，C. glabrata）中转录辅激活因子Ada2所调控的基因，我们通过RNA测序比较了野生型菌株CBS138与ada2突变体的全基因组表达谱。经分析，相较于野生型菌株，ada2突变体中共有43个基因表达下调、443个基因表达上调。上述43个下调基因涵盖麦角固醇生物合成通路（ERG6、ERG13）、黏附素或类黏附素基因（ICS2、AWP2、EPA7）、热休克基因（HSP30、SSB2）及其他细胞功能相关基因。在443个上调基因中，包含9个类黏附素EPA家族基因（EPA1、EPA6、EPA8、EPA10、EPA11、EPA12、EPA15、EPA20、EPA23）与3个yapsin家族基因（YPS4、YPS8、YPS10）。本研究对提取自光滑念珠菌野生型CBS138与ada2突变体的总mRNA进行了RNA测序分析。