A microglia-CD4+ T cell partnership generates protective anti-tumor immunity to glioblastoma [RNA-seq]

The aim of the study is to analyze and compare the transcriptional profiles of tumor-infiltrating Microglia from glioma brearing brain tissues treated with anti-CTLA4 in the presence or absence of CD4 T cells . RNA of all Microglia from Glioma-005 glioblastoma tumors treated with IgG, anti_CTLA4 and combination of anti_CTLA4 and anti_CD4 (depletion) were performed at Day 35 post implantation.

本研究旨在分析并对比经抗CTLA4（anti-CTLA4）处理、且存在或缺失CD4 T细胞（CD4 T cells）的荷胶质瘤脑组织中肿瘤浸润小胶质细胞（tumor-infiltrating Microglia）的转录谱。本研究对经IgG、抗CTLA4以及抗CTLA4联合抗CD4（细胞耗竭）处理的Glioma-005胶质母细胞瘤来源的全部小胶质细胞的RNA进行了检测，样本采集于肿瘤移植后第35天。