Salmonella wild type and mutant sdiA in LB and 2mM indole. Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

Transcriptional profiling of Salmonella Typhimurium SL1344 wild type and deletion of the sdiA regulator-encoding gene grown to OD600=1 in LB alone or in LB supplemented with 2mM indole. The goal is to determine Salmonella response to indole and to define the SdiA-regulated genes in these conditions. Overall design: 4 different conditions tested (wild type in LB, wild type in LB + 2mM indole, sdiA mutant in LB, sdiA mutant in LB + 2mM indole), 4 replicates for each condition. The reference on each hybridisation is the chromosomal DNA of the wild type strain.

本数据集针对鼠伤寒沙门氏菌（Salmonella Typhimurium）SL1344野生型及其sdiA调控因子编码基因缺失株展开转录谱分析，上述菌株均在仅含LB培养基或添加2mM吲哚的LB培养基中培养至光密度600nm处吸光度值（OD600）为1。本研究旨在明确沙门氏菌对吲哚的应答反应，并界定上述培养条件下受SdiA调控的基因。实验总体设计：共设置4种不同培养条件（LB培养基中的野生型菌株、添加2mM吲哚的LB培养基中的野生型菌株、LB培养基中的sdiA缺失突变株、添加2mM吲哚的LB培养基中的sdiA缺失突变株），每种条件设置4次生物学重复。所有杂交实验的参照样本均为野生型菌株的染色体DNA。