Leaves of 7 accessions of pepper infected by GFP-tagged Potato virus Y, from 5dpi to 22 dpi

Photographs of leaves inoculated with a Green Fluorescent Protein (GFP) tagged Potato virus Y. Leaves are from 7 accessions of pepper (Capsicum annuum L.), inoculated by aphid transmission (I), mock inoculated (M) or healthy controls (TS). Fluorescence progression was tracked through regular photography at 5, 7, 8, 11, 13, 15, 18, 20, and 22 dpi. Images were captured using a Nikon D5300 digital camera set to aperture F8, ISO 2000, and a shutter speed of 1/2 second, with a picture size of 4496 × 3000 pixels. Fluorescence photographs were captured using a photo bench from the manufacturer BLS (Budapest, Hungary), equipped with lamps designed for GFP excitation, with an emission spectrum ranging from 460 to 495 nm. A green filter with a bandwidth between 500 and 515 nm was mounted on the camera lens to minimize background light and selectively capture GFP emission. A low-intensity white light source was added to allow differentiation of healthy tissues from the background. Focusing was performed under intense white light prior to each photograph; this light was then turned off before the image was captured. The fabric background was selected for its high light-absorption properties to reduce reflections and enhance image clarity.

本数据集收录了接种绿色荧光蛋白（Green Fluorescent Protein, GFP）标记马铃薯Y病毒（Potato virus Y）的叶片图像。供试叶片取自7份辣椒（Capsicum annuum L.）种质，分别接受蚜虫传毒接种（I组）、模拟接种（M组）处理，或作为健康对照（TS组）。研究人员于接种后5、7、8、11、13、15、18、20及22天（days post inoculation，以下简称dpi）通过定期拍摄追踪荧光信号的动态变化。常规明场图像采集使用尼康D5300数码相机，参数设置为光圈F8、ISO 2000、快门速度1/2秒，单张图像分辨率为4496×3000像素。荧光图像则采用匈牙利布达佩斯BLS公司生产的摄影成像平台拍摄，该平台配备适配GFP激发的专用光源，其发射光谱范围为460~495 nm；相机镜头加装带宽为500~515 nm的绿色滤光片，以降低背景光干扰并选择性捕获GFP的发射荧光。为实现健康组织与拍摄背景的区分，系统额外搭载低强度白光源；每张图像拍摄前，先使用强光完成对焦，随后关闭该强光再进行图像采集。背景采用高吸光性织物材质，以减少反射、提升图像整体清晰度。