Time-course of HCoV-229E infection of A549 cells. Homo sapiens

The aim of the experiment is to identify kinetic changes in host gene expression upon infection of A549 lung epithelial carcinoma cells with human corona virus HCoV-229E as compared to unifected cells. Negativ controls include infections with heat-inactivated virus. Positive controls include treatment of cells with human IL-1alpha (10ng/ml) for 1h. All experiments have been performed at 33°C which is required for sufficient viral entry/replication. Overall design: Single-color hybridizations of RNA from HCoV.229E infected A549 cells, time course (16,24,48 h), with time-mathched controls plus two additional controls (untreated and IL1-treated). No replicates.

本实验旨在探究人冠状病毒HCoV-229E感染A549肺上皮癌细胞后宿主基因表达的动力学变化，并以未感染细胞作为对照。阴性对照设置为热灭活病毒感染组，阳性对照为以10ng/ml人白细胞介素1α（IL-1α）处理细胞1小时的组别。所有实验均在33℃下开展，该温度是病毒实现有效入侵与复制的必要条件。实验整体设计：对HCoV-229E感染的A549细胞的RNA进行单通道杂交，设置16、24、48小时三个时间点的时间梯度，配备对应时间点的对照组，外加未处理组与IL-1α处理组两个额外对照。本实验未设置重复。