Additional file 3 of Potential and expression of carbohydrate utilization by marine fungi in the global ocean

Additional file 2: Figure S1. Distribution of samples and niche differentiation of genes encoding fungal CAZymes categorized by “depth” (top plots) and by “size” (lower plots). Principal coordinate analysis (PCoA) of fungal CAZyme genes present in the metagenome (left plots) and metatranscriptome (right plots). Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Figure S2. Correlation of the occurrence of the metagenome and metatranscriptome of genes encoding fungal CAZymes for the macro-mycobiome (left) and micro-mycobiome (right). P-values and R2 provided in the plots. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Figure S3. Taxonomic affiliation of genes (A) and transcripts (B) encoding fungal CAZymes at the class level. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Each bar represents a sample collected in each of the stations/location and depth; so that missing bars (empty white space) represents stations/locations where samples were not collected at that particular depth. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic; IO, Indian Ocean; MS, Mediterranean Sea; NAO, North Atlantic Ocean; North Pacific Ocean; SAO, South Atlantic Ocean; SO, Southern Ocean; SPO, South Pacific Ocean. Figure S4. Taxonomic affiliation of genes (A) and transcripts (B) encoding secretory fungal CAZymes at the phylum level. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Each bar represents a sample collected in each of the stations/location and depth; so that missing bars (empty white space) represents stations/locations where samples were not collected at that particular depth. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic; IO, Indian Ocean; MS, Mediterranean Sea; NAO, North Atlantic Ocean; North Pacific Ocean; SAO, South Atlantic Ocean; SO, Southern Ocean; SPO, South Pacific Ocean. Figure S5. Taxonomic affiliation of genes (A) and transcripts (B) encoding secretory fungal CAZymes at the class level. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Each bar represents a sample collected in each of the stations/location and depth; so that missing bars (empty white space) represents stations/locations where samples were not collected at that particular depth. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic; IO, Indian Ocean; MS, Mediterranean Sea; NAO, North Atlantic Ocean; North Pacific Ocean; SAO, South Atlantic Ocean; SO, Southern Ocean; SPO, South Pacific Ocean. Figure S6. Functional classification of genes (A) and transcripts (B) encoding secretory fungal CAZymes. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Each bar represents a sample collected in each of the stations/location and depth; so that missing bars (empty white space) represents stations/locations where samples were not collected at that particular depth. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic; IO, Indian Ocean; MS, Mediterranean Sea; NAO, North Atlantic Ocean; North Pacific Ocean; SAO, South Atlantic Ocean; SO, Southern Ocean; SPO, South Pacific Ocean. Figure S7. Occurrence of genes and transcripts for fungal CAZymes targeting different carbohydrate sources. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2,000 μm). Box shows median and interquartile range (IQR); whiskers show 1.5 × IQR of the lower and upper quartiles or range; outliers extend to the data range. Statistics are based on a Wilcoxon test, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, not significant. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic. Note that carbohydrates originating from bacterial (peptidoglycan) detritus were not plotted because they were not found in the metagenome or metatranscriptomes of pelagic fungi. Figure S8. Occurrence of genes and transcripts for secretory fungal CAZymes targeting different carbohydrate sources. Micro, micro-mycobiome (0.8-5 μm); Macro, macro-mycobiome (5-2000 μm). Box shows median and interquartile range (IQR); whiskers show 1.5 × IQR of the lower and upper quartiles or range; outliers extend to the data range. Statistics are based on Wilcoxon test, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, not significant. SRF, surface; MXL, mixed layer; DCM, deep chlorophyll maximum; MES, mesopelagic.

补充材料2：图S1。按“深度”（上图）与“粒径”（下图）分类的真菌编码碳水化合物活性酶（Carbohydrate-Active enZYmes, CAZymes）基因的采样分布与生态位分化。针对宏基因组（左图）与宏转录组（右图）中存在的真菌CAZymes基因的主坐标分析（Principal Coordinate Analysis, PCoA）。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。
图S2。针对宏真菌组（左图）与微真菌组（右图）的真菌编码CAZymes基因，其宏基因组与宏转录组的出现频率相关性。图中给出了P值与决定系数R²。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。
图S3。纲水平下真菌编码CAZymes的基因（A）与转录本（B）的分类归属。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。每个柱状条代表各站位/采样点与深度下采集的一份样本；缺失的柱状条（空白区域）代表该深度未采集样本的站位/采样点。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带；IO，印度洋；MS，地中海；NAO，北大西洋；North Pacific Ocean，北太平洋；SAO，南大西洋；SO，南大洋；SPO，南太平洋。
图S4。门水平下真菌编码分泌型CAZymes的基因（A）与转录本（B）的分类归属。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。每个柱状条代表各站位/采样点与深度下采集的一份样本；缺失的柱状条（空白区域）代表该深度未采集样本的站位/采样点。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带；IO，印度洋；MS，地中海；NAO，北大西洋；North Pacific Ocean，北太平洋；SAO，南大西洋；SO，南大洋；SPO，南太平洋。
图S5。纲水平下真菌编码分泌型CAZymes的基因（A）与转录本（B）的分类归属。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。每个柱状条代表各站位/采样点与深度下采集的一份样本；缺失的柱状条（空白区域）代表该深度未采集样本的站位/采样点。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带；IO，印度洋；MS，地中海；NAO，北大西洋；North Pacific Ocean，北太平洋；SAO，南大西洋；SO，南大洋；SPO，南太平洋。
图S6。真菌编码分泌型CAZymes的基因（A）与转录本（B）的功能分类。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。每个柱状条代表各站位/采样点与深度下采集的一份样本；缺失的柱状条（空白区域）代表该深度未采集样本的站位/采样点。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带；IO，印度洋；MS，地中海；NAO，北大西洋；North Pacific Ocean，北太平洋；SAO，南大西洋；SO，南大洋；SPO，南太平洋。
图S7。靶向不同碳水化合物底物的真菌CAZymes的基因与转录本出现情况。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。箱线图的箱体代表中位数与四分位距（IQR）；须线代表上下四分位数的1.5×IQR范围或数据全距；异常值延伸至数据实际范围。统计检验采用Wilcoxon检验，*P<0.05，**P<0.01，***P<0.001，****P<0.0001，ns，无显著性差异。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带。注：源自细菌（肽聚糖）碎屑的碳水化合物未被纳入绘图，因为远洋真菌的宏基因组与宏转录组中未检测到此类底物相关的CAZymes。
图S8。靶向不同碳水化合物底物的真菌分泌型CAZymes的基因与转录本出现情况。Micro，微真菌组（0.8~5 μm）；Macro，宏真菌组（5~2000 μm）。箱线图的箱体代表中位数与四分位距（IQR）；须线代表上下四分位数的1.5×IQR范围或数据全距；异常值延伸至数据实际范围。统计检验采用Wilcoxon检验，*P<0.05，**P<0.01，***P<0.001，****P<0.0001，ns，无显著性差异。SRF，表层；MXL，混合层；DCM，深层叶绿素最大值层；MES，中层浮游带。