Effects of mating on gene expression profiles of mature ant queens

Will be added/updated once the manuscript is finalized. Cardiocondyla obscurior queens. Three treatments: virgin queens, queens mated by real males, queens sham-mated (by sterile males). Queens collected 1 week and 8 week after mating. seven loops for queens collected 1 week after mating; nine loops for queens collected 8 weeks after mating. Five direct comparisons (with dye-swaps... so 10 arrays) were done of between 1 week and 8 week samples of queens mated by real males. Each sample is RNA from two queens (from different colonies). Samples were hybridized against Solenopsis invicta microarrays (signal was detectable for most clones!)

稿件最终定稿后将补充/更新本说明。本数据集涉及暗胸弓背蚁（Cardiocondyla obscurior）蚁后样本，共设置3组处理：处女蚁后、与正常雄蚁交配的蚁后、经绝育雄蚁假交配的蚁后。分别于交配后1周和8周采集蚁后样本；其中交配后1周采集的样本对应7次芯片杂交循环，交配后8周采集的样本对应9次芯片杂交循环。针对与正常雄蚁交配的蚁后，分别对其交配后1周和8周的样本开展了5组直接对比实验（包含染料交换实验，因此共使用10张基因芯片）。每份样本均提取自2只来自不同蚁群的蚁后的核糖核酸（RNA）。所有样本均与红火蚁（Solenopsis invicta）基因芯片进行杂交，多数克隆均可检测到杂交信号！