Additional file 1: Supplementary Tables. of Multiple gene movements into and out of haploid sex chromosomes

Table S1. Genes present in the male and female sex-specific region of the Ectocarpus sp. sex chromosome. Table S2. Strains used in this study, sequencing statistics and accession numbers. Table S3. Synonymous nucleotide substitutions (dS) for gametologue pairs in the brown algae included in this study. Table S4. Molecular evolutionary features and transcript levels of Ectocarpus sp. and S. japonica sex-linked genes. Table S5. PAML codeml analysis for evidence of positive selection acting on SDR genes using sequences from three Ectocarpus species and S. lomentaria. Table S6. Sex-linked genes and their autosomal homologues in Ectocarpus sp. (Esp), S. lomentaria (Slom), S. japonica (Sjap), M. pyrifera (Mpyr) and U. pinnatifida (Upin). Table S7. Orphan retrogenes in S. japonica for which the parental copy homologue is still present in Ectocarpus sp. genome. Table S8. Nucleotide and protein sequence of the orthologs of the Ectocarpus sp. male and female SDR genes recovered from E. siliculosus, E. fasciculatus, S. lomentaria, S. japonica, M. pyrifera and U. pinnatifida. Table S9. Strains of S. japonica used in this study to correlate phenotypic and genotypic sex. Table S10. Primers used to determine sex linkage in S. japonica. Table S11 Assessment of phenotypic and genotypic sex in S. japonica individuals. (XLSX 318 kb)

表S1 水云属未定种（Ectocarpus sp.）性染色体雌雄性别特异性区域所含基因
表S2 本研究所用菌株、测序统计数据及序列登录号
表S3 本研究涉及的褐藻配子同源基因对的同义核苷酸替换率（dS）
表S4 水云属未定种（Ectocarpus sp.）与海带（Saccharina japonica, S. japonica）的性连锁基因的分子进化特征及转录水平
表S5 利用3个水云属物种及长囊藻（S. lomentaria）的序列，通过PAML的codeml程序分析性别决定区（Sex-determining region, SDR）基因所受正向选择的证据
表S6 水云属未定种（Ectocarpus sp.，缩写Esp）、长囊藻（S. lomentaria，缩写Slom）、海带（Saccharina japonica，缩写Sjap）、巨藻（Macrocystis pyrifera，缩写Mpyr）及裙带菜（Undaria pinnatifida，缩写Upin）的性连锁基因及其常染色体同源基因
表S7 海带（S. japonica）中的孤儿逆转录基因，其亲本拷贝同源基因仍存在于水云属未定种（Ectocarpus sp.）基因组中
表S8 从丝状水云（Ectocarpus siliculosus, E. siliculosus）、簇生水云（Ectocarpus fasciculatus, E. fasciculatus）、长囊藻（S. lomentaria）、海带（S. japonica）、巨藻（Macrocystis pyrifera, M. pyrifera）及裙带菜（Undaria pinnatifida, U. pinnatifida）中获取的水云属未定种（Ectocarpus sp.）雌雄性别决定区（SDR）基因的直系同源序列的核苷酸与蛋白质序列
表S9 本研究中用于关联表型性别与基因型性别的海带（S. japonica）菌株
表S10 用于检测海带（S. japonica）性连锁特征的引物
表S11 海带（S. japonica）个体的表型性别与基因型性别鉴定结果（XLSX格式，文件大小318 KB）