Effects of sleep deprivation on susceptibility to parasitic infection

We investigate the role of sleep deprivation on Drosophila nigrospiracula susceptibility to parasitic infections by the ectoparasitic mite, Macrocheles subbadius. We tested the hypothesis that sleep deprivation reduces behavioural resistance against mites, resulting in higher rates of infection among sleep deprived (SD) flies compared to non-sleep deprived flies, and that this is mediated by lower endurance (in negative geotaxis assays) among SD flies. We tested the impact of sleep deprivation on susceptibility to infection and climbing endurance in two age groups, flies 14 and 21 days post-eclosion. , In the first experiment, flies from two age classes (14 and 21 days old) were subject to sleep deprivation during a 12 hr night period and then tested for susceptibility to infection. Control flies were left undisturbed for the night. For a given age class, five flies from each of treatment group were placed inside an infection chamber containing mites and left for 2 hours. Flies were then removed from the chamber and assessed for mite infection and number of mites. In a second experiment, flies were again subject to sleep deprivation or left undisturbed for a period of 12 hrs. Flies were then transferred to vials for negative geotaxis assays. Flies were placed inside a vial with a line located 5 cm from the base, once the fly climbed up to the line the vial was tapped to knock the fly down to the bottom. We measured climbing endurance as the number of times (cycles) and total duration a fly spent climbing. , , # Effects of sleep deprivation on susceptibility to parasitic infection

[https://doi.org/10.5061/dryad.fqz612k37](https://doi.org/10.5061/dryad.fqz612k37)

## Description of the data and file structure

**Infection data:**

Flies from two age class (14 and 21 days old) were subject to sleep deprivation for 12 hours; control flies were left undisturbed. After 12 hrs, 5 flies from each treatment group (sleep deprived and control) were placed inside infection chambers (jars) containing ectoparasitic mites. After 2 hours, flies were removed and the number of mites on the flies was recorded. The experimented was replicated over time (blocks). Infection status was recorded as yes(1) or no(0) and \"mites\" = the number of mites on an infected fly.

**Endurance data:**

21-days old flies from were subject to sleep deprivation for 12 hours; control flies were left undisturbed. After 12 hrs, flies were transferred transferred to individual vials with a line marked at 5 cm from the bottom of the vi...

我们研究了睡眠剥夺对黑螺旋果蝇（Drosophila nigrospiracula）感染外寄生螨亚巴德巨螯螨（Macrocheles subbadius）易感性的影响。我们验证了以下假设：睡眠剥夺会降低果蝇对螨类的行为抗性，导致睡眠剥夺（SD）组果蝇的感染率高于非睡眠剥夺组；且这种效应由SD组果蝇较低的耐力（通过负趋地性实验（negative geotaxis assays）测定）所介导。我们在两个年龄组（羽化后14天和21天的果蝇）中测试了睡眠剥夺对感染易感性和攀爬耐力的影响。在第一个实验中，两个年龄组（14天和21天）的果蝇在12小时夜间时段接受睡眠剥夺处理，随后测试其感染易感性；对照组果蝇则不受干扰。对于每个年龄组，将处理组的5只果蝇置于含螨的感染室中2小时，之后取出果蝇并评估其螨感染情况及螨数量。在第二个实验中，果蝇再次接受12小时睡眠剥夺或不受干扰处理，随后转移至试管进行负趋地性实验。将果蝇置于底部上方5cm处标有刻度线的试管中，当果蝇爬至刻度线时轻敲试管使其落回底部，我们以攀爬次数（循环数）和总持续时间来衡量攀爬耐力。

# 睡眠剥夺对寄生感染易感性的影响

[https://doi.org/10.5061/dryad.fqz612k37]

## 数据及文件结构描述

**感染数据：**

两个年龄组（14天和21天）的果蝇接受12小时睡眠剥夺处理，对照组果蝇不受干扰。12小时后，将每个处理组（睡眠剥夺组和对照组）的5只果蝇置于含外寄生螨的感染室（ jars）中。2小时后取出果蝇，记录其身上的螨数量。实验随时间重复（分批次）。感染状态记录为是（1）或否（0），“螨数”=受感染果蝇身上的螨数量。

**耐力数据：**

21天龄的果蝇接受12小时睡眠剥夺处理，对照组果蝇不受干扰。12小时后，将果蝇转移至底部上方5cm处标有刻度线的独立试管中……