Brucella abortus delta bab1_1186 (EipB) Tn-seq data

The goal of this study was to define a set of genes in a Brucella abortus strain deleted for gene bab1_1186 (eipB) that cannot be disrupted by a Tn-Himar transposon. A comparison of this dataset to a Tn-seq dataset from wild-type B. abortus identifies genes that are synthetically lethal with gene eipB. Cells were cultured axenically in a complex medium and mutagenized using the barcoded Tn system described in Wetmore et al, 2015; doi: 10.1128/mBio.00306-15. Data contain the end of the transposon, with barcode, and reveal the junction between the transposon and the B. abortus chromosome.

本研究旨在明确一株敲除bab1_1186（eipB）基因的流产布鲁氏菌（Brucella abortus）菌株中，无法被Tn-Himar转座子（Tn-Himar transposon）插入破坏的基因集合。将本数据集与野生型流产布鲁氏菌的Tn-seq（转座子测序）数据集进行比对，可鉴定出与eipB基因存在合成致死效应的基因。实验菌株于复合培养基中进行体外纯培养，并采用Wetmore等2015年报道的带条形码转座子系统完成诱变处理（DOI: 10.1128/mBio.00306-15）。本数据集包含带有条形码的转座子末端序列，并揭示了转座子与流产布鲁氏菌染色体之间的连接位点。