C6 glioblastoma microRNA regulation induced by haloperidol, risperidone and clozapine. C6 glioblastoma microRNA regulation induced by haloperidol, risperidone and clozapine

Antipsychotic drugs (APDs) have been reported to ease psychotic symptoms in the clinic. miRNAs have also been identified that can regulate the expression of genes/proteins related to possible molecular mechanisms of drug action. To reveal APD-induced miRNA modulations in glial cells, we analyzed miRNA expression in subchronically APD-treated C6 cells by using a miRNA microarray. Overall design: C6 cells were subchronically treated with haloperidol, risperidone or clozapine for five days. The final concentrations of haloperidol (2 μg/ml), risperidone (4 μg/ml) and clozapine (25 μg/ml) were calculated based on therapeutic concentrations in clinic. Cells were then harvested for microarray analysis. miRNA expression of APD-treated C6 cells were compared to miRNA expression of untreated control C6 cells. The microarray experiments were performed in two biological repeats.

抗精神病药物（Antipsychotic drugs, APDs）已被证实可在临床中缓解精神病性症状。另有研究发现，微小RNA（microRNA, miRNAs）能够调控与药物作用潜在分子机制相关的基因/蛋白表达。为揭示抗精神病药物诱导的神经胶质细胞内微小RNA表达调控变化，本研究通过微小RNA芯片技术分析了经亚慢性抗精神病药物处理的C6细胞内的微小RNA表达水平。
整体实验设计：将C6细胞经氟哌啶醇、利培酮或氯氮平进行亚慢性处理，持续时长为5天。氟哌啶醇、利培酮及氯氮平的最终作用浓度分别为2 μg/ml、4 μg/ml与25 μg/ml，该浓度参考临床治疗浓度换算得到。随后收集细胞用于芯片分析，将抗精神病药物处理组C6细胞的微小RNA表达谱与未处理的对照组C6细胞进行对比。本芯片实验设置2次生物学重复。