Summary of adult lifespan assays in various mutant backgrounds.

All the assays were carried out at 20°C and on regular NGM plates. For Set #4, assays were carried out on plates containing FuDR (50 µg/ml) to avoid the internal hatching of the asm-3(ok1744);age-1(mg305) mutant animals. Each experiment was individually grouped and statistical analyses were carried out for the experiment data set and the wild-type control data set in each group (P values). In addition, statistical analyses were carried out for the survival data of the assayed single mutant (marked as *) and the corresponding double mutant containing the asm-3(ok1744) allele (*P values). In Set #6, P value between asm-3(ok1744) and asm-3(ok1744);akt-1(mg306) is 0.064. Each set of the lifespan experiments was repeated at least two independent times and similar results were obtained. Data from representative sets of experiments are shown. Greater than 50 worms were counted for each strain in each experiment.

所有实验均于20℃条件下在常规NGM平板上完成。针对第4组实验，为避免asm-3(ok1744);age-1(mg305)突变体动物出现体内孵化现象，实验在添加了50 μg/ml FuDR的平板上进行。每项实验均单独分组，并对每组内的实验数据集与野生型对照数据集开展统计学分析，同步报告P值。此外，还对经检测的单突变体（标记为*）以及携带asm-3(ok1744)等位基因的对应双突变体的存活数据进行统计学分析，双突变体的P值以*标注。在第6组实验中，asm-3(ok1744)与asm-3(ok1744);akt-1(mg306)两组间的P值为0.064。每组寿命实验均至少独立重复两次，且获得了一致的实验结果。本研究展示了代表性实验组的实验数据。每项实验中，每个供试菌株均统计了至少50条线虫。