Definitive Screening Design to Improve Data-Independent MS Analysis of Neuropeptides

A definitive screening design was used to systematically optimize a DIA workflow for crustacean neuropeptide quantitation. We were able to assess several parameters for their effect on increasing quantifiable neuropeptides and predict the optimal value for these parameters.

本研究采用确定性筛选设计（definitive screening design），针对甲壳类动物神经肽定量分析的数据非依赖采集（Data-Independent Acquisition，DIA）工作流开展系统性优化。我们能够评估多个参数对提升可定量神经肽数量的影响，并预测这些参数的最优取值。